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RNAseq Workflow
Illumina Tru-Seq RNA-seq protocol
RNA-seq (RNA-sequencing) is a technique that can examine the quantity and sequences of RNA in a sample using next generation sequencing (NGS). It analyzes the transcriptome of gene expression patterns encoded within our RNA.
Our current workflow includes:
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Pre-process the raw high-throughput sequencing data.
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Produce gene-level count matrices using normalising offsets.
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Find statistically significant, differentially expressed genes.
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Perform pathway and gene ontology analyses.
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Generate different visualisations of the results.
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