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Single-cell RNAseq Workflow

10x ChromiumTM Single Cell 3’ Solution

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Single-cell RNA sequencing (scRNA-seq) technologies allow the dissection of gene expression at single-cell resolution, which greatly revolutionizes transcriptomic studies. A number of scRNA-seq protocols have been developed, and these methods possess their unique features with distinct advantages and disadvantages.

Our current workflow includes: 

  • Pre-process the raw high-throughput sequencing data.

  • Feature selection

  • Dimensionality reduction

  • Clustering and assigning identity 

  • Differential expression tests

  • Perform pathway and gene ontology analyses.

  • Generate different visualisations of the results.

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