top of page

RNAseq Workflow

Illumina Tru-Seq RNA-seq protocol

RNAseq.jpg

RNA-seq (RNA-sequencing) is a technique that can examine the quantity and sequences of RNA in a sample using next generation sequencing (NGS). It analyzes the transcriptome of gene expression patterns encoded within our RNA. 

Our current workflow includes: 

  • Pre-process the raw high-throughput sequencing data.

  • Produce gene-level count matrices using normalising offsets.

  • Find statistically significant, differentially expressed genes.

  • Perform pathway and gene ontology analyses.

  • Generate different visualisations of the results.

bottom of page